Life Science Journal of Pakistan <p>Life Science Journal of Pakistan (LSJP) is an open-access peer-review journal that is published quarterly. Journal is free and there is no fee for processing or publishing of manuscripts. The journal publishes original articles, review articles, letters to editors, case reports, or short communications technical notes and perspectives. LSJP provides immediate open access to its content so that making research freely available to the public supports a greater global exchange of knowledge which is essential to promote research in the life science domain. The journal publishes contents related to the biology of living things. It may include subjects from biochemistry, microbiology, zoology, plant science, agriculture science, biotechnology, molecular biology, medical sciences, epidemiology, and food science. </p> en-US (Yasar Saleem) (Muhammad Shaheen Iqbal) Tue, 07 Jul 2020 00:00:00 -0400 OJS 60 Applications and perspectives of chitosan as functional biopolymer; an extended review <p>Chitosan, a deacetylated derivative of chitin, has gained high importance for its nontoxicity, biocompatibility, biodegradability and antioxidant properties. The main objective of this review is to explore various applications of chitosan in different fields of life. It is used in food preservatives or coating material in natural origin due to its antimicrobial activity and film forming prpoerties. Chitosan-based coatings are made up by the combination of chitosan with organic, inorganic or with non-coating materials which includes gas fumigation, heat treatment and hypobaric treatment. It is used in various biomedical applications such as wound dressings, antibacterial coatings, tissue engineering scaffolds, drug delivery, stent coatings, biosensors and membrane seprations. Chitosan is a biopolymer which have different functional properties including enhacnced gelling properties, treatment of seafood industry effluent, drug compounds from aqua culture and sea food and nanocarrier abilities for bioactive compounds. Chitosan is also used for nasal formulations because of its efficient mucoadhesive properties including greater convenience, stability and long-term availability of drug in nasal cavity. Chitosan and its derivatives are used in nanocarriers for drug/ gene delivery or other healthcare purposes, due to their efficient hydrophilic properties.</p> Muhammad Jahangeer, Sarmad Ahmad Qamar, Zahed Mahmood, Muhammad Asgher Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400 Matricaria Chamomile Protective Effect Against Type-2 Diabetes Mellitus and Cancer Cell Linings Shahnai Basharat, Ameer Hamza, Sammana Ghazanfer, Amna Imtiaz, Sanabil Anmol, Mian Anjum Murtaza, Muhammad Mustafa Qamar, Faiza Iftikhar, Syed Amir Gilani, Affifa Rajpoot Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400 List of Contents Issue 2 Volume 1 Muhammad Shaheen Iqbal Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400 Editorial Issue 2 Volume 1 <p>Editorial for Issue 2 Volume 1 by Chief Editor LSJP</p> Mushtaq Saleem Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400 Correlation of blaNDM to Antibiotic Resistance Patterns in Klebsiella pneumoniae Isolates from Tertiary Care Hospital in Lahore, Pakistan <p>This study was conducted to evaluate the antibiotic susceptibility of <em>Klebsiella pneumoniae</em> isolated from different clinical samples collected at a Tertiary Care Hospital in Lahore, Pakistan. A total of 50 isolates were included in this study and 28 different antibiotics were used to determine the antibiotic susceptibility of isolates. The pure colonies of <em>K. pneumoniae </em>were isolated by conventional method of bacterial identification and then the pure bacterial growths were tested for antibiotic susceptibility against different antibiotics. PCR amplification was performed to identify three virulent genes; <em>Klebsiella</em><em> pneumoniae</em> Carbapenamase (KPC), Metallo-?-lactamases (NDM), Imipenamase (IMP) followed by sequencing analysis. The <em>K. pneumoniae </em>isolates were found highly resistant to Ampicillin by 56% and Amoxicillin, Cefepime, Cefixime, Cefaclor, and Cefuroxime by 54%. The isolates were highly sensitive to Colistin (100%), Amikacin (14%), Imipenem and Meropenem (18%). The prevalence of antibiotic resistance was found more in female patients as compared to males. The prevalence of NDM gene was 22% while we were unable to detect KPC and IMP in these isolates. In the present study, a prominent correlation was found between the presence of NDM gene and antibiotic resistance as this gene was found positive only in MDR strains. The co-existence of multiple drug resistance and the NDM gene is an alarming situation and demand more research to determine possible mechanism of co-existence between blaNDM and antibiotic resistance.</p> Pakeeza Munir Bhatti, Basit Zeshan, Mushtaq Ahmad Saleem, Maida Qureshi, Sadaf Riaz Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400 Isolation and partial characterization of antimicrobial proteins /peptides from Moringa oleifera Anum Shakir, Sadaf Riaz, Muhammad Afzal, Basit Zeshan, Salma Batool Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400 In-Silico Analysis of Grapevine Germin like Protein (VvGLP3) and its Probable Role in Defense Against Powdery Mildew Disease <p>Germin like proteins (GLPs) is a heterogeneous group of proteins that are part of Cupin superfamily. Various GLPs of Grapevine (VvGLP3) have been identified and characterized at molecular but not at the structural level. This study analyzes the structure of VvGLP3 to elucidate its role in defense against powdery mildew disease caused by <em>Erysiphe necator</em>. Physiochemical analysis through the high aliphatic index (AI), low instability index and grand average of hydropathy (GRAVY) reveal that VvGLP3 is heat stable hydrophobic protein. InterProScan was used to check the similarity of VvGLP3 to other GLPs and TMHMM indicates that VvGLP3 is a transmembrane protein. Secondary structure of VvGLP3 reveals that it consists of random coils followed by extended ?-helix and ?-trends and I-TASSER was employed to construct a 3-D structure of VvGLP3. A molecular docking approach was applied to study molecular interaction and binding pattern by Auto Dock Vina. I-TASSER and SOPMA were used to determine the functionally important residues and their role. In the present study, molecular docking binding affinity energy is from -1.5 to -1.3 of germin like protein with manganese (Mn<sup>+2</sup>) co-factor which depict its role in defense against powdery mildew disease.</p> Aqeel Ahmad, Abubakar Ahmed, Ramsha Essa, Shanzay Baber, Arooj Jamshed, Almeera Shoukat, Tasleem Younas, Mushtaq A. Saleem, Muhammad Naveed Copyright (c) 2019 Life Science Journal of Pakistan Tue, 24 Sep 2019 00:00:00 -0400